Tapentadol Rapid Test

Intended Use

Tapentadol Rapid Test (Urine) is a rapid visual immunoassay for the qualitative, presumptive detection of Tapentadol in human urine specimens at the cut-off concentrations.

Introduction

Tapentadol is a centrally acting opioid analgesic of the benzenoid class with a dual mode of action as anagonist of the μ-opioid receptor and as a norepinephrine reuptake inhibitor. Analgesia occurs within 32 minutes of oral administration, and lasts for 4-6 hours. It is similar to tramadol in its dual mechanism ofaction; namely, its ability to activate the mu opioid receptor and inhibit the reuptake of norepinephrine.

Principle

Tapentadol Rapid Test (Urine) detects Tapentadol through visual interpretation of color development on the strip. Drug conjugates are immobilized on the test region of the membrane. During testing, the specimen reacts with antibodies conjugated to colored particles and precoated on the sample pad. The mixture then migrates through the membrane by capillary action, and interacts with reagents on the membrane. If there are insufficient drug molecules in the specimen, the antibody-colored particle conjugate will bind to the drug conjugates, forming a colored band at the test region of the membrane. Therefore, a colored band appears in the test region when the urine is negative for the drug. If drug molecules are present in the urine above the cut-off concentration of the test, they compete with the immobilized drug conjugate on the test region for limited antibody binding sites. This will prevent attachment of the antibody-colored particle conjugate to the test region. Therefore, the absence of a colored band at the test region indicates a positive result. The appearance of a colored band at the control region serves as a procedural control, indicating that the proper volume of specimen has been added and membrane wicking has occurred.

Test Procedure

The TAP Rapid Test Strip (Urine)

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