Mugwort Allergy Test

Intended Use

Mugwort Allergy Test is a rapid test for the qualitative determination of Mugwort specific Immunoglobulin E (sIgE) in human serum, plasma or whole blood.

The test, in conjunction with other clinical observations, is intended to identify the patient whose allergic symptoms may be mediated by Mugwort -specific immunoglobulin E (IgE) Type I hypersensitivity.

PRINCIPLE

Mugwort Allergy Test has been designed to detect Mugwort sIgE through visual interpretation of color development in the internal strip. The membrane was immobilized with streptavidin on the test region, the conjugate pad was pre-coated with colored anti-IgE antibody colloidal gold conjugates and the sample pad was pre-coated with biotinylated Mugwort Protein. After specimens were added, the gold-conjugates move along the membrane chromatographically by capillary action and antibodies get to the test region. If sufficient Mugwort sIgE is present in the sample, it will react with biotinylated Mugwort protein in sample pad, the mixture then migrates through conjugate pad by capillary action and interact with colored anti-IgE antibody colloidal gold conjugates, form a complex. Then the complex moves to the membrane, and combine with streptavidin. As a result, a colored band will form at the test region of the membrane.

If no Mugwort sIgE is present in the sample, biotinylated allergen pre-coated on the sample pad will bind to streptavidin immediately, so there is no colored line at the test region of the membrane.

Therefore, the colored band on the test region indicates a positive result. And appearance of a colored band at the control region serves as a procedural control. This indicates that proper volume of specimen has been added and membrane wicking has occurred.

Test Procedure

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