C-Reactive Protein Rapid Test

Intended use

C-Reactive Protein Rapid Test (Whole Blood/ Serum/ Plasma) is used for semi-quantitative determination and monitoring of CRP concentrations in whole blood/ serum/ plasma specimens. C-Reactive Protein Rapid Test is based on two specific antibodies against human CRP.

What is CRP?

C-reactive Protein (CRP) in patient’s serum has been found in association with acute infections, necrotic conditions and a variety of inflammatory disorders. There is a strong correlation between serum levels of CRP and the onset of the inflammatory process. Monitoring the levels of CRP in patient’s serum indicates the effectiveness of treatment and the assessment of patient recovery. It is used in particular to differentiate bacterial infections from virus infections.

Specimen Collection and Storage

Preparation

Before performing the test, please make sure that all components are brought to room temperature (see storage). Take a vial with buffer solution out of the kit. Mark with patient’s name or ID. Open the screw cap.

Blood Sample Taking

1) Disinfect the fingertip. Use lancet device extract a drop of blood from the finger tip:

2) With the supplied capillary, take a volume of 10 μL from the blood drop. It is important that the end-to-end capillary is filled until the upper end. Due to hygienic reasons, hold the capillary with a capillary holder or tweezers. Alternatively, the blood can also be taken with a micro pipette. Please note: In case of using micropipettes or other capillaries, a sample volume of exactly 10 μL must be administered.

Please dilute the blood sample immediately to avoid clotting.

Sample Dilution / Sample Stability

3) Administer the blood-filled end-to-end capillary into the accordingly plastic vial with dilution buffer. Alternatively, the 10 μL of blood can be added directly with the micro pipette into the buffer. OR transfer 5 μL serum/plasma into the accordingly plastic vial with dilution buffer.

4) Close the vial and shake the sample by hand forcefully for approximately 10 seconds so that the blood is released from the capillary and sample and dilution buffer mix themselves well.

5) Let the diluted sample rest for approximately 1 minute.

6) The sample can then be used immediately or stored for up to 8 hours.

NOTE:

EDTA-, citrate- or heparin- blood can be used as well. Before performing the test, it has to be diluted accordingly with the supplied buffer.

Test Procedure

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